Abstract
Two new alkaloids, hypserpanines A and B (1, 11), together with eleven known compounds, phenolbetain (2), acutumine (3), acutumidine (4), dechloroacutumine (5), dauricumine (6), dauricumidine (7), pronuciferine (8), glaziovine (9), S-reticuline (10), magnoflorine (12) and laurifoline(13), were isolated from Hypserpa nitida Miers. (Menispermaceae) and chemically elucidated through spectral analyses. All the isolated alkaloids were evaluated for their anti-HBV activities in vitro using the HBV transfected Hep G2.2.15 cell line. The most active compound, dauricumidine (7), exhibited an IC(50) value of 0.450 mM (SI=4.13) on hepatitis B virus (HBV) surface antigen (HBsAg) secretion of the Hep G2.2.15 cell line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / chemistry
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Alkaloids / pharmacology*
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Antiviral Agents / chemistry
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Antiviral Agents / pharmacology*
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Cell Line
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Chromatography, Ion Exchange
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Diethylamines / chemistry
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Diethylamines / pharmacology*
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Hepatitis B Surface Antigens / metabolism
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Hepatitis B e Antigens / metabolism
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Hepatitis B virus / drug effects*
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Heterocyclic Compounds, 3-Ring / chemistry
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Heterocyclic Compounds, 3-Ring / pharmacology*
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Humans
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Magnetic Resonance Spectroscopy
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Menispermaceae / chemistry*
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Models, Molecular
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Molecular Conformation
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Spectrometry, Mass, Electrospray Ionization
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Spiro Compounds / chemistry
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Spiro Compounds / pharmacology*
Substances
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Alkaloids
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Antiviral Agents
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Diethylamines
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Hepatitis B Surface Antigens
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Hepatitis B e Antigens
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Heterocyclic Compounds, 3-Ring
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Spiro Compounds
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hypserpanine A
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hypserpanine B