Cytoprotective constituent of Hoveniae Lignum on both Hep G2 cells and rat primary hepatocytes

Arch Pharm Res. 2007 Jun;30(6):674-7. doi: 10.1007/BF02977626.

Abstract

A phytochemical investigation of the EtOH extract of Hoveniae Lignum yielded four phenolic compounds, phloretin (1), 5-(4'-hydroxyphenyl)-gamma-valerolactone (2), (-)-epiafzelechin (3), and maesopsin (4). Compound 1 was hepatoprotective against tacrine-induced cytotoxicity in human liver-derived Hep G2 cells with an EC50 value of 37.55 +/- 0.42 microM. Compound 1 (0.4-200 microM) also significantly reduced tert-butyl hydroperoxide-induced cytotoxicity in rat primary hepatocytes as measured by the cellular leakage of lactate dehydrogenase and the level of aspartate transaminase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Aspartate Aminotransferases / metabolism
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Cholinesterase Inhibitors / toxicity
  • Hepatocytes / drug effects*
  • Humans
  • L-Lactate Dehydrogenase / metabolism
  • Magnetic Resonance Spectroscopy
  • Male
  • Mass Spectrometry
  • Phloretin / pharmacology
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Rats
  • Rats, Sprague-Dawley
  • Rhamnaceae / chemistry*
  • Spectrophotometry, Ultraviolet
  • Tacrine / antagonists & inhibitors
  • Tacrine / toxicity
  • tert-Butylhydroperoxide / toxicity

Substances

  • Antioxidants
  • Cholinesterase Inhibitors
  • Plant Extracts
  • Tacrine
  • tert-Butylhydroperoxide
  • L-Lactate Dehydrogenase
  • Aspartate Aminotransferases
  • Phloretin