Dissolved inorganic carbon (DIC), pH, the partial pressure of CO (pCO2), total alkalinity (TA), dissolved oxygen saturation (DO), chlorophyll a (Chl-a), and NH4+, PO4(3-) were studied in mixing zone of Yellow River estuary from September 2004 to April 2006. The results indicate that pH value is higher than that of freshwater in low salinity area where DIC is non-conservative. NH4+, PO4(3-) sharply decreasing indicates that biogenic respiration is possibly inhibited in early mixing of freshwater and seawater, then pCO2, which is derived from biogenic respiration, swiftly decrease and lead to CaCO3 precipitation in low salinity area, and the pH value will simultaneously increase in this area. Instant increasing pH value can be used as index of CaCO3 precipitation in Yellow River estuary. According to dissolved matter mixing model in estuary, the observation of 1:1 removal of TA and DIC provides just evidence that DIC removal we observed in the field is a result of CaCO3 precipitation. The process can clean 10% (1.21 x 10(5) tons) DIC which is transported by freshwater both in dry and wet weathers, and the annually effective DIC flux to sea of Yellow River is 10.86 x 10(5) tons. Ten percent of CO2 (2.24 x 10(5) t) which is absorbed by chemical weathering process in drainage basin will release to atmosphere in Yellow River estuary.