Comparison of hand-held test kits, immunofluorescence microscopy, enzyme-linked immunosorbent assay, and flow cytometric analysis for rapid presumptive identification of Yersinia pestis

H Tomaso; P Thullier; E Seibold; V Guglielmo; A Buckendahl; L Rahalison; H Neubauer; H C Scholz; W D Splettstoesser

doi:10.1128/JCM.00458-07

Comparison of hand-held test kits, immunofluorescence microscopy, enzyme-linked immunosorbent assay, and flow cytometric analysis for rapid presumptive identification of Yersinia pestis

J Clin Microbiol. 2007 Oct;45(10):3404-7. doi: 10.1128/JCM.00458-07. Epub 2007 Jul 25.

Authors

H Tomaso¹, P Thullier, E Seibold, V Guglielmo, A Buckendahl, L Rahalison, H Neubauer, H C Scholz, W D Splettstoesser

Affiliation

¹ Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. herberttomaso@bundeswehr.org

Abstract

An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent benchtop tools.

Publication types

Comparative Study

MeSH terms

Bacterial Proteins / analysis*
Enzyme-Linked Immunosorbent Assay / methods*
Flow Cytometry / methods*
Microscopy, Fluorescence
Reagent Kits, Diagnostic*
Yersinia pestis / isolation & purification*

Substances

Bacterial Proteins
Reagent Kits, Diagnostic
caf1 protein, Yersinia pestis