Ribavirin (RV) (1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide), is a synthetic purine nucleoside analog with a broad spectrum of antiviral activity. To better understand the mechanism of action of RV, as well as its pharmacokinetic characteristics, an assay that can allow specific, sensitive, and accurate measurement of RV in biologic samples is critical. In this way, diverse analytical methods have been established. In this work, we have recompiled these methods with the aim to present the different options for the RV determination.