Repeated fractionation of the acetylated methanol extract of Prasium majus afforded acetylated derivatives of a new iridoid glycoside hamighriprasin (1), myoporoside (2), beta-D-glycopyranose (3) and saccharose (4). The structures of 1 and 2 were established by interpretation of 1D and 2D NMR data of their corresponding acetylated derivatives and by comparison with the literature.