Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples

Panagiotis Karanis; Oriel Thekisoe; Klytaimnistra Kiouptsi; Jerry Ongerth; Ikuo Igarashi; Noborou Inoue

doi:10.1128/AEM.01152-07

Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of cryptosporidium oocysts in fecal and water samples

Appl Environ Microbiol. 2007 Sep;73(17):5660-2. doi: 10.1128/AEM.01152-07. Epub 2007 Jul 6.

Authors

Panagiotis Karanis¹, Oriel Thekisoe, Klytaimnistra Kiouptsi, Jerry Ongerth, Ikuo Igarashi, Noborou Inoue

Affiliation

¹ National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Hokkaido 080-8555, Japan. Panagiotis.Karanis@uk-koeln.de

Abstract

A loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidemiologic studies of Cryptosporidium presence.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Cryptosporidium / genetics
Cryptosporidium / growth & development
Cryptosporidium / isolation & purification*
DNA Probes
DNA, Protozoan / analysis*
DNA, Protozoan / isolation & purification
Feces / parasitology*
Humans
Nucleic Acid Amplification Techniques / methods*
Oocysts / chemistry*
Rivers / parasitology*
Sensitivity and Specificity

Substances

DNA Probes
DNA, Protozoan