Abstract
A non-invasive orthotopic hepatocellular carcinoma (HCC) model was created with human HCC cells (HepG-Luc) constitutively expressing luciferase (Luc) in nude mice. Development of tumor growth and response to anti-tumor therapy combined with 5-fluorouracil and cisplatin was monitored by whole-body bioluminescent imaging (BLI). Luciferase activity in the tumor, determined by BLI, correlated with the tumor volume and weight. The anti-tumor therapy proved effective by BLI monitoring. In conclusion, BLI by luciferase provides a non-invasive method of monitoring tumor activities that can prove useful for therapeutic intervention studies.
MeSH terms
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Animals
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Antimetabolites, Antineoplastic / administration & dosage
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Antineoplastic Combined Chemotherapy Protocols / therapeutic use
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Carcinoma, Hepatocellular / drug therapy
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Carcinoma, Hepatocellular / enzymology*
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Diagnostic Imaging / methods
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Disease Models, Animal
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Endpoint Determination
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Female
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Fluorouracil / administration & dosage
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Humans
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Liver Neoplasms, Experimental / drug therapy
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Liver Neoplasms, Experimental / enzymology*
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Luciferases / analysis*
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Luciferases / metabolism
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Mice
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Mice, Inbred BALB C
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Treatment Outcome
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Xenograft Model Antitumor Assays
Substances
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Antimetabolites, Antineoplastic
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Luciferases
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Fluorouracil