Combined therapy of the antimetastatic compound NAMI-A and electroporation on B16F1 tumour cells in vitro

Ajda Bicek; Iztok Turel; Masa Kanduser; Damijan Miklavcic

doi:10.1016/j.bioelechem.2007.05.002

Combined therapy of the antimetastatic compound NAMI-A and electroporation on B16F1 tumour cells in vitro

Bioelectrochemistry. 2007 Nov;71(2):113-7. doi: 10.1016/j.bioelechem.2007.05.002. Epub 2007 Jun 2.

Authors

Ajda Bicek¹, Iztok Turel, Masa Kanduser, Damijan Miklavcic

Affiliation

¹ Faculty of Electrical Engineering, University of Ljubljana, Trzaska 25, SI-1000 Ljubljana, Slovenia.

PMID: 17602896
DOI: 10.1016/j.bioelechem.2007.05.002

Abstract

Ruthenium complex NAMI-A [ImH][trans-RuCl(4)(DMSO-S)Im] (Im = imidazole) is a potential chemotherapeutic drug in cancer treatment. Electroporation can be used to facilitate delivery of NAMI-A into cells. Suspension of B16F1 tumour cells from mouse melanoma in NAMI-A solution was exposed to a train of electric pulses. The effect of NAMI-A was determined by examining cell viability in clonogenic test. Our results show that electroporation increases the otherwise scarce in vitro effects of NAMI-A, i.e. reduces cell viability. At the conditions chosen for experiments 90% of cells survived in the presence of 1 microM NAMI-A, whereas in a combined treatment with 1 microM NAMI-A and electroporation only about 10% of cells survived.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / therapeutic use*
Antineoplastic Agents / toxicity
Cell Line, Tumor
Cell Survival / drug effects
Dimethyl Sulfoxide / analogs & derivatives*
Dimethyl Sulfoxide / therapeutic use
Dimethyl Sulfoxide / toxicity
Electrochemotherapy*
Electroporation
Melanoma, Experimental / drug therapy*
Melanoma, Experimental / pathology*
Mice
Neoplasm Metastasis / drug therapy
Organometallic Compounds / therapeutic use*
Organometallic Compounds / toxicity
Ruthenium Compounds

Substances

Antineoplastic Agents
Organometallic Compounds
Ruthenium Compounds
imidazolium-bis(imidazole)dimethylsulfoxideimidazotetrachlororuthenate(III)
Dimethyl Sulfoxide