Mass spectrometric studies on mouse hippocampal synapsins Ia, IIa, and IIb and identification of a novel phosphorylation site at serine-546

Julius Paul Pradeep John; Wei-Qiang Chen; Arnold Pollak; Gert Lubec

doi:10.1021/pr070157r

Mass spectrometric studies on mouse hippocampal synapsins Ia, IIa, and IIb and identification of a novel phosphorylation site at serine-546

J Proteome Res. 2007 Jul;6(7):2695-710. doi: 10.1021/pr070157r. Epub 2007 Jun 19.

Authors

Julius Paul Pradeep John¹, Wei-Qiang Chen, Arnold Pollak, Gert Lubec

Affiliation

¹ Department of Pediatrics, Medical University of Vienna, Waehringer Guertel 18, 1090 Vienna, Austria.

PMID: 17579389
DOI: 10.1021/pr070157r

Abstract

Synapsins are key phosphoproteins in the mammalian brain, and structural research on synapsins is still holding center stage. Proteins were extracted from hippocampal tissue and separated on two-dimensional gel electrophoresis (2-DE), and the spots were analyzed by MALDI-TOF-TOF and nano-LC-ESI-MS/MS. Synapsins Ia, IIa, and IIb were unambiguously identified and represented by 15 individual spots on 2-DE. Several serine phosphorylation sites were confirmed, and a novel phosphorylation site was observed at Ser-546 in synapsin IIa in all gels analyzed.

MeSH terms

Acetylation
Alternative Splicing
Amino Acid Sequence
Animals
Electrophoresis, Gel, Two-Dimensional
Hippocampus / chemistry*
Male
Methionine / chemistry
Methylation
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Nanotechnology / methods
Phosphoproteins / chemistry*
Phosphorylation
Phosphoserine / analysis*
Protein Conformation
Protein Isoforms / chemistry
Protein Isoforms / genetics
Serine / chemistry*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
Synapsins / chemistry*
Synapsins / genetics

Substances

Phosphoproteins
Protein Isoforms
Synapsins
Phosphoserine
Serine
Methionine