Two multiplex real-time TaqMan polymerase chain reaction systems for simultaneous detecting and serotyping of Ureaplasma parvum

Diagn Microbiol Infect Dis. 2007 Sep;59(1):109-11. doi: 10.1016/j.diagmicrobio.2007.04.014. Epub 2007 Jun 15.

Abstract

Studies of epidemiology and pathogenesis of infections with Ureaplasma have received much attention in recent years. Rapid molecular methods for serotyping of Ureaplasma parvum will be of great value in the studies. We developed 2 multiplex real-time TaqMan polymerase chain reaction systems that allow simultaneous detecting and serotyping of 4 serovars of U. parvum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Probes / genetics
  • Female
  • Genotype
  • Humans
  • Polymerase Chain Reaction / methods
  • Pregnancy
  • Serotyping / methods*
  • Taq Polymerase
  • Ureaplasma / classification*
  • Ureaplasma / genetics
  • Ureaplasma / isolation & purification
  • Ureaplasma urealyticum / classification*
  • Ureaplasma urealyticum / genetics
  • Ureaplasma urealyticum / isolation & purification
  • Vagina / microbiology

Substances

  • DNA Probes
  • Taq Polymerase