DNA sequence analysis of a cryptic plasmid pL2 from Lactococcus lactis subsp. lactis

Biotechnol Lett. 2007 Oct;29(10):1519-27. doi: 10.1007/s10529-007-9418-2. Epub 2007 Jun 12.

Abstract

A cryptic plasmid pL2 was isolated from Lactococcus lactis subsp. lactis and its complete nucleotide sequence was determined (5,299 bp, GenBank accession No. DQ917780). Its replication mode was identified as a theta-type belonging to the pAM beta 1 family. Analysis of the nucleotide sequence revealed that pL2 contained a transfer origin, a replication origin, and five putative open reading frames (ORF1-5). ORF1 (386 amino acids) was homologous to replication protein RepB. The other open reading frames were cryptic. A small counter-transcript RNA (ctRNA) coding region was also observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Blotting, Southern
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Lactococcus lactis / genetics*
  • Molecular Sequence Data
  • Open Reading Frames / genetics
  • Plasmids / chemistry
  • Plasmids / genetics*
  • Sequence Analysis, DNA / methods*

Substances

  • Bacterial Proteins
  • DNA, Bacterial

Associated data

  • GENBANK/DQ917780