With the completion of human genome sequence project (HGP), the application of two-dimensional gel electrophoresis (2-DE), mass spectrometry (MS), and bioinformatics, more achievements have been made in proteomics research. From the new point of view, looking for tumor-specific proteins with proteomics research strategy may help to elucidate the correlation of the change of protein expression to oncogenesis, and may play a vital role in the early detection of cancer and anticancer therapy including drug exploitation. This article briefly reviewed the relevant techniques, strategies, and application of proteomics research on lung cancer.