The method of determination of indicative flavonoids of propolis has been developed. The method is applicable for the standardization and estimation of quality and origin of raw propolis and propolis-containing food products, food dietary supplements (FDS) and drugs. The method allows to determine the concentration and composition of individual flavonoids on the background of complicated matrix of food products and FDS. The detection limit amounted 0, 1% (for chrysin). 20 samples of propolis from different regions and 12 samples of propolis-enriched food products and FDS were investigated by proposed method.