Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice

Miyako Kusano; Atsushi Fukushima; Makoto Kobayashi; Naomi Hayashi; Pär Jonsson; Thomas Moritz; Kaworu Ebana; Kazuki Saito

doi:10.1016/j.jchromb.2007.05.002

Application of a metabolomic method combining one-dimensional and two-dimensional gas chromatography-time-of-flight/mass spectrometry to metabolic phenotyping of natural variants in rice

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Aug;855(1):71-9. doi: 10.1016/j.jchromb.2007.05.002. Epub 2007 May 16.

Authors

Miyako Kusano¹, Atsushi Fukushima, Makoto Kobayashi, Naomi Hayashi, Pär Jonsson, Thomas Moritz, Kaworu Ebana, Kazuki Saito

Affiliation

¹ RIKEN Plant Science Center, Yokohama, Kanagawa, Japan. mkusano005@psc.riken.jp

PMID: 17556050
DOI: 10.1016/j.jchromb.2007.05.002

Abstract

We have developed a comprehensive method combining analytical techniques of one-dimensional (1D) and two-dimensional (GC x GC) gas chromatography-time-of-flight (TOF)-mass spectrometry. This method was applied to the metabolic phenotyping of natural variants in rice for the 68 world rice core collection (WRC) and two other varieties. Ten metabolites were selected as metabolite representatives, and the selected ion current of each metabolite peak obtained from both techniques were statistically compared. Our method of combining 1D- and GC x GC-TOF/MS is useful for the metabolic phenotyping of natural variants in rice for further studies in breeding programs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gas Chromatography-Mass Spectrometry / methods*
Oryza / chemistry
Oryza / classification
Oryza / metabolism*
Phenotype