Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

Qijie Chen; Junko Kon; Hidekazu Ooe; Kazunori Sasaki; Toshihiro Mitaka

doi:10.1038/nprot.2007.118

Selective proliferation of rat hepatocyte progenitor cells in serum-free culture

Nat Protoc. 2007;2(5):1197-205. doi: 10.1038/nprot.2007.118.

Authors

Qijie Chen¹, Junko Kon, Hidekazu Ooe, Kazunori Sasaki, Toshihiro Mitaka

Affiliation

¹ Department of Pathophysiology, Cancer Research Institute, Sapporo Medical University School of Medicine, South-1, West-17, Chuo-Ku, Sapporo 060-8556, Japan.

PMID: 17546015
DOI: 10.1038/nprot.2007.118

Abstract

This protocol details a method of obtaining selectively proliferated hepatocyte progenitor cells using hyaluronic acid (HA)-coated dishes and serum-free medium. A small hepatocyte (SH) is a hepatocyte progenitor cell of adult livers and has many hepatic functions. When the rat SH begins to proliferate, CD44 is specifically expressed. To define the purification of SH, CD44 and cytokeratin 8 are used as marker proteins. The growth of SHs is faster on HA-coated dishes than on other extracellular matrix-coated ones. The use of both DMEM/F12 medium and HA-coated dishes allows the selective proliferation of SHs in culture. The purification of SHs is approximately 85% at day 10.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Proliferation*
Hepatocytes / cytology*
Hyaluronan Receptors / metabolism
Hyaluronic Acid
Keratin-8
Rats
Stem Cells / cytology*

Substances

Hyaluronan Receptors
Keratin-8
Hyaluronic Acid