Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance

Jared Castor; Linda Cook; Lawrence Corey; Keith R Jerome

doi:10.1128/JCM.00526-07

Rapid detection directly from patient serum samples of human cytomegalovirus UL97 mutations conferring ganciclovir resistance

J Clin Microbiol. 2007 Aug;45(8):2681-3. doi: 10.1128/JCM.00526-07. Epub 2007 May 30.

Authors

Jared Castor¹, Linda Cook, Lawrence Corey, Keith R Jerome

Affiliation

¹ Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, USA.

Abstract

Ganciclovir-resistant cytomegalovirus can cause disease and death in transplant recipients. We describe here a rapid PCR- and sequencing-based assay for ganciclovir resistance that can be performed in 1 to 2 working days directly from patient specimens, without the need for amplification of the virus by cell culture. An evaluation of 120 sequential samples submitted for clinical testing revealed a variety of silent and amino acid mutations.

Publication types

Evaluation Study

MeSH terms

Antiviral Agents / pharmacology*
Cytomegalovirus / drug effects*
Cytomegalovirus / genetics
Cytomegalovirus Infections / virology
Drug Resistance, Viral / genetics*
Ganciclovir / pharmacology*
Humans
Microbial Sensitivity Tests / methods*
Mutation, Missense
Phosphotransferases (Alcohol Group Acceptor) / genetics*
Polymerase Chain Reaction / methods
Sequence Analysis, DNA
Serum / virology*

Substances

Antiviral Agents
Phosphotransferases (Alcohol Group Acceptor)
ganciclovir kinase
Ganciclovir