A metabonomic study of strain- and age-related differences in the Zucker rat

Rapid Commun Mass Spectrom. 2007;21(13):2039-45. doi: 10.1002/rcm.3059.

Abstract

Ultra-performance liquid chromatography (UPLC) coupled to orthogonal acceleration time-of-flight mass spectrometry (oa-TOFMS) in positive electrospray ionization mode was used to obtain metabolite profiles for urine obtained from three strains of Zucker rat. These were the Zucker lean, the Zucker (fa/fa) obese and the Zucker lean/(fa) cross. Clear age- and strain-related differences were noted with the leptin-deficient (fa/fa) obese animal showing significant differences from both the other Zucker rat strains with respect to metabolite profiles.

Publication types

  • Comparative Study

MeSH terms

  • Age Factors*
  • Animals
  • Chromatography, Liquid
  • Crosses, Genetic
  • Mass Spectrometry
  • Obesity / genetics*
  • Rats
  • Rats, Zucker / genetics*
  • Rats, Zucker / metabolism*
  • Rats, Zucker / urine
  • Spectrometry, Mass, Electrospray Ionization
  • Thinness / genetics*
  • Time Factors