Inhibitory effects of glycitein on hydrogen peroxide induced cell damage by scavenging reactive oxygen species and inhibiting c-Jun N-terminal kinase

Free Radic Res. 2007 Jun;41(6):720-9. doi: 10.1080/10715760701241618.

Abstract

The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H(2)O(2)) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H(2)O(2) treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H(2)O(2) induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Apoptosis / drug effects*
  • Blotting, Western
  • Cells, Cultured / drug effects
  • Cells, Cultured / metabolism
  • Comet Assay
  • Cricetinae
  • Cytoprotection
  • Electrophoretic Mobility Shift Assay
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Flow Cytometry
  • Free Radical Scavengers / pharmacology
  • Hydrogen Peroxide / pharmacology*
  • Isoflavones / metabolism
  • Isoflavones / pharmacology*
  • JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors*
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Luciferases / metabolism
  • Lung / cytology
  • Lung / drug effects
  • Lung / metabolism
  • Mitogen-Activated Protein Kinase 9 / metabolism
  • Oxidants / pharmacology*
  • Oxidative Stress
  • Phytoestrogens / pharmacology
  • Promoter Regions, Genetic / genetics
  • Reactive Oxygen Species / metabolism*
  • Transcription Factor AP-1 / genetics
  • Transcription Factor AP-1 / metabolism

Substances

  • Antioxidants
  • Free Radical Scavengers
  • Isoflavones
  • Oxidants
  • Phytoestrogens
  • Reactive Oxygen Species
  • Transcription Factor AP-1
  • glycitein
  • Hydrogen Peroxide
  • Luciferases
  • Mitogen-Activated Protein Kinase 9
  • Extracellular Signal-Regulated MAP Kinases
  • JNK Mitogen-Activated Protein Kinases
  • glycitin