Abstract
The present study investigated the cytoprotective properties of glycitein, a metabolite formed by the transformation of glycitin by intestinal microflora, against oxidative stress. Glycitein was found to scavenge intracellular reactive oxygen species (ROS), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and thereby preventing lipid peroxidation and DNA damage. Glycitein inhibited apoptosis of Chinese hamster lung fibroblast (V79-4) cells exposed to hydrogen peroxide (H(2)O(2)) via radical scavenging activity. Glycitein abrogated the activation of c-Jun N-terminal kinase (JNK) induced by H(2)O(2) treatment and inhibited DNA binding activity of activator protein-1 (AP-1), a downstream transcription factor of JNK. Taken together, these findings suggest that glycitein protected H(2)O(2) induced cell death in V79-4 cells by inhibiting ROS generation and JNK activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antioxidants / pharmacology
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Apoptosis / drug effects*
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Blotting, Western
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Cells, Cultured / drug effects
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Cells, Cultured / metabolism
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Comet Assay
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Cricetinae
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Cytoprotection
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Electrophoretic Mobility Shift Assay
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Extracellular Signal-Regulated MAP Kinases / metabolism
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Flow Cytometry
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Free Radical Scavengers / pharmacology
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Hydrogen Peroxide / pharmacology*
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Isoflavones / metabolism
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Isoflavones / pharmacology*
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JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors*
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JNK Mitogen-Activated Protein Kinases / metabolism
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Luciferases / metabolism
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Lung / cytology
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Lung / drug effects
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Lung / metabolism
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Mitogen-Activated Protein Kinase 9 / metabolism
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Oxidants / pharmacology*
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Oxidative Stress
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Phytoestrogens / pharmacology
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Promoter Regions, Genetic / genetics
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Reactive Oxygen Species / metabolism*
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Transcription Factor AP-1 / genetics
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Transcription Factor AP-1 / metabolism
Substances
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Antioxidants
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Free Radical Scavengers
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Isoflavones
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Oxidants
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Phytoestrogens
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Reactive Oxygen Species
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Transcription Factor AP-1
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glycitein
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Hydrogen Peroxide
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Luciferases
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Mitogen-Activated Protein Kinase 9
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Extracellular Signal-Regulated MAP Kinases
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JNK Mitogen-Activated Protein Kinases
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glycitin