The effect of the different combination system of constructed wetland on zooplankton community structure was studied with different hydraulic loading [0.6 m3 x (m2 x d)(-1), 0.8 m3 x (m2 x d)(-1), 1.0 m3 x (m2 x d)(-1]. And the seasonal change of the effect was measured with the same hydraulic loading. The results show that different combination system with hydraulic loading 0.8 m3 x (m2 x d)(-1) on zooplankton community structure is more effective than others; the effect in spring, summer and autumn are more notable than in winter, otherwise the effect in autumn is the most notable; the effect on macro-zooplankton is more obvious than on micro-zooplankton and there are some species in the effluent water of wetland but not in influent water. Ponds of the systems weaken the effects on zooplankton community structure and the effect of combination system (down-flow --> aerobic and anaerobic pond) is the worst of all combination system, and in contrast to others down-flow --> up-flow --> horizontal-flow wetland and down-flow --> horizontal-flow wetland present distinct effects on zooplankton.