NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants

Nucleic Acids Res. 2007 Jul;35(Web Server issue):W66-70. doi: 10.1093/nar/gkm305. Epub 2007 May 8.

Abstract

The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at http://oblab.cs.nchu.edu.tw:8080/WebSDL/.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Automation
  • DNA Primers*
  • DNA, Complementary / metabolism*
  • Gene Deletion
  • Humans
  • Internet
  • Mutagenesis*
  • Mutation*
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Single Nucleotide*
  • Sequence Analysis, DNA
  • Software*
  • User-Computer Interface

Substances

  • DNA Primers
  • DNA, Complementary