Preparative isolation and purification of triterpene saponins from Clematis mandshurica by high-speed counter-current chromatography coupled with evaporative light scattering detection

J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Jun 1;852(1-2):679-83. doi: 10.1016/j.jchromb.2007.02.056. Epub 2007 Mar 19.

Abstract

Preparative high-speed counter-current chromatography (HSCCC) coupled with evaporative light scattering detection (ELSD) was successfully applied to the isolation and purification of four triterpene saponins from Clematis mandshurica by one-step separation. The solvent system was composed of ethyl acetate-n-butanol-ethanol-0.05% TFA (5:10:2:20, v/v). The purities of all the saponins obtained were better than 97%. The structures of the compounds were elucidated by the means of spectroscopic methods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Chromatography, High Pressure Liquid / methods
  • Clematis / chemistry*
  • Countercurrent Distribution / methods*
  • Light
  • Molecular Sequence Data
  • Reference Standards
  • Saponins / chemistry
  • Saponins / isolation & purification*
  • Scattering, Radiation
  • Triterpenes / chemistry
  • Triterpenes / isolation & purification*

Substances

  • Saponins
  • Triterpenes