Increased coverage in the transmembrane domain with activated-ion electron capture dissociation for top-down Fourier-transform mass spectrometry of integral membrane proteins

J Proteome Res. 2007 Jun;6(6):2205-10. doi: 10.1021/pr0607031. Epub 2007 Apr 19.

Abstract

The c-subunit of ATP synthase (AtpH) is an 8 kD integral membrane protein with two transmembrane domains; we set out to demonstrate it amenable to top-down electrospray-ionization Fourier-transform mass spectrometry (FT-MS) using both collision activated and electron capture dissociation (CAD/ECD). Thermal activation concomitant with electron delivery was necessary for efficient ECD (activated-ion ECD; aiECD), yielding complementary information and greater sequence coverage in the transmembrane domains in comparison with CAD.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / chemistry
  • Electrons
  • Ions
  • Membrane Proteins / analysis*
  • Mitochondrial Proton-Translocating ATPases / analysis*
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Proteomics / methods*
  • Spectrometry, Mass, Electrospray Ionization / methods*

Substances

  • Ions
  • Membrane Proteins
  • mitochondrial ATPase subunit c
  • Mitochondrial Proton-Translocating ATPases