Abstract
Promoters of nine Bacillus subtilis genes (bcrC, yacK, ydaH, yfnI, yjbD, ypbG, ypuA, yraA, and ysxA), all responsive to artificially induced increases in the stress-responsive extracytoplasmic function sigma factor, SigM, were mapped by rapid amplification of cDNA ends-PCR. The resulting promoter consensus suggests that overlapping control by SigX or SigW is common.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Artificial Gene Fusion
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Bacillus subtilis / genetics
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Bacillus subtilis / physiology*
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Base Sequence
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Gene Expression Regulation, Bacterial / genetics
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Gene Expression Regulation, Bacterial / physiology*
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Genes, Bacterial*
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Genes, Reporter
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Polymerase Chain Reaction
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Promoter Regions, Genetic*
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Regulon / genetics
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Regulon / physiology
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Sequence Alignment
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Sigma Factor / genetics
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Sigma Factor / physiology*
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beta-Galactosidase / biosynthesis
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beta-Galactosidase / genetics
Substances
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Sigma Factor
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beta-Galactosidase