Oxygen is an environmental and developmental signal regulator, and its role is involved in energy homeostasis, development and process of differentiation. Myoblasts persist in skeletal muscle as satellite cells, which possess capability of self-renewing and differentiation into mature myofiber. Myoblasts play a critical role in postnatal muscle regeneration after injury as well as maintaining myofibers' function. Though oxygen is vital to nearly all forms of life, studies focused on investigating the effects of oxygen level on proliferation and differentiation of myoblasts are few. Lower oxygen concentration is more close to the level of oxygen in physiological and pathological environment in vivo. So physiological environment is actually optimum condition for myogenesis. It is significant for understanding repair and regeneration of skeletal muscle to study on effects of hypoxia on myogenesis. HIF-1 signaling pathway was involved in these processes as well as other signaling pathways would be, and accordingly, deep studying and further revealing the signaling pathways involved in mechanism will provide evidences or references for looking for novel targets for stem cells therapy and drug treatment.