Red seabream iridovirus (RSIV) disease is a serious disease of many marine fish species in Japan and elsewhere. For a better understanding of the molecular pathogenic mechanism, we examined the transcriptional profile of RSIV in infected fish using a DNA microarray. Expression of RSIV open reading frames (ORFs) was first detected at about 5 days post-infection (d.p.i.), and accounted for about 45% of total ORFs. Almost all the ORFs (97-99%) were expressed at their maximum levels during 7-9 d.p.i. The expression levels and the number of expressed ORFs started to decrease at 10 d.p.i. These results suggest that pathogenesis of RSIV infection began at around day 5, and continued with high levels of viral multiplication until viral clearance by host antiviral defenses starting from around 10 d.p.i. A comparison of viral gene expressions in the spleen and kidney over the course of the infection suggests that RSIV preferentially targets the spleen. The spleen may thus be the most susceptible organ for diagnosis of iridoviral disease.