Cooperation of group 2 sigma factors, SigD and SigE for light-induced transcription in the cyanobacterium Synechocystis sp. PCC 6803

Tsutomu Yoshimura; Sousuke Imamura; Kan Tanaka; Makoto Shirai; Munehiko Asayama

doi:10.1016/j.febslet.2007.03.010

Cooperation of group 2 sigma factors, SigD and SigE for light-induced transcription in the cyanobacterium Synechocystis sp. PCC 6803

FEBS Lett. 2007 Apr 3;581(7):1495-500. doi: 10.1016/j.febslet.2007.03.010. Epub 2007 Mar 13.

Authors

Tsutomu Yoshimura¹, Sousuke Imamura, Kan Tanaka, Makoto Shirai, Munehiko Asayama

Affiliation

¹ Laboratory of Molecular Genetics, School of Agriculture, Ibaraki University, 3-21-1 Ami, Inashiki, Ibaraki 300-0393, Japan.

PMID: 17379215
DOI: 10.1016/j.febslet.2007.03.010

Abstract

A light-inducible sigma factor of RNA polymerase, SigD, can contributes to the light-induced transcription of psbA in the cyanobacterium Synechocystis sp. PCC 6803. Here, another light-induced sigma factor, SigE, was characterized together with SigD. Results indicated that SigE also contributes to light-induced transcription on the cpcBACD, psbA, petBD and psaAB promoters whose potential sequences are of the Escherichia coli sigma(70)-type. SigD and SigE interfere with each other's expression. A rhythmic expression, in which the periodic peak of SigE exhibits a 24-h interval according to the upcoming night, was observed at the protein level. The cooperation of group 2 sigma factors, SigD and SigE, for light-induced transcription was discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Regulation, Bacterial*
Genes, Bacterial / genetics
Light*
Promoter Regions, Genetic / radiation effects
Sigma Factor / metabolism*
Synechocystis / genetics*
Synechocystis / radiation effects
Transcription, Genetic / radiation effects*

Substances

Sigma Factor