Polymerase chain reaction detection of Clostridium perfringens in feces from captive and wild chimpanzees, Pan troglodytes

J Med Primatol. 2007 Feb;36(1):25-32. doi: 10.1111/j.1600-0684.2006.00191.x.

Abstract

Background: For veterinary management of non-human primates in captivity, and conservation of wild-living primates, management of their health risks is necessary. Incidences of pathogenic bacteria in the fecal specimens are considered as one of the useful indicators for non-invasive health monitoring.

Methods: We carried out the detection of Clostridium perfringens in feces from captive and wild chimpanzees by the rapid polymerase chain reaction method.

Results: The bacterium was detected in most fecal specimens (80%) in captive chimpanzees. Contrarily, the detection rate in the wild chimpanzees was low, with 23% (n = 12) of 53 fecal samples from the Bossou group, Guinea, and 1.2% (1/81) in the Mahale group, Tanzania.

Conclusions: These results show that the intestinal microflora differs between Pan populations under various living conditions, being influenced by their diet and environment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Wild / microbiology
  • Clostridium perfringens / isolation & purification*
  • Feces / microbiology*
  • Guinea
  • Pan troglodytes / microbiology*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Tanzania