Abstract
Background:
Adult T-cell leukemia (ATL) is an acute malignancy of activated T-cells caused by the human T-cell lymphotrophic virus type-1 (HTLV-1).
Materials and methods:
The effects of non-cytotoxic concentrations of ascorbic acid (AA) were evaluated against HTLV-1 positive and negative cells. The effect of AA on apoptosis and proliferation was evaluated by cell cycle analysis. The role of p53, p21 Bax and Bcl-2a on cell cycle modulation and apoptosis was also assessed. The anti-proliferative effects were tested by determining the changes in the expression of transforming growth factors (TGF-alpha, TGF-beta1 and TGF-beta2).
Results:
Ascorbic acid was found to reduce the proliferation of cells and induce apoptosis by the modulation of p53, p21, Bcl-2 and Bax.
Conclusion:
The results of this study show the anti-proliferative effects of AA against leukemic cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Ascorbic Acid / pharmacology*
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Cell Cycle / drug effects
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Cell Growth Processes / drug effects
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Cyclin-Dependent Kinase Inhibitor p21 / biosynthesis
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Enzyme-Linked Immunosorbent Assay
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Flow Cytometry
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HTLV-I Infections / complications
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HTLV-I Infections / drug therapy*
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HTLV-I Infections / pathology
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Human T-lymphotropic virus 1*
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Humans
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Jurkat Cells
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Leukemia-Lymphoma, Adult T-Cell / drug therapy*
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Leukemia-Lymphoma, Adult T-Cell / metabolism
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Leukemia-Lymphoma, Adult T-Cell / pathology
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Leukemia-Lymphoma, Adult T-Cell / virology
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Proto-Oncogene Proteins c-bcl-2 / biosynthesis
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Transforming Growth Factors / biosynthesis
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Tumor Suppressor Protein p53 / biosynthesis
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bcl-2-Associated X Protein / biosynthesis
Substances
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BAX protein, human
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CDKN1A protein, human
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Cyclin-Dependent Kinase Inhibitor p21
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Proto-Oncogene Proteins c-bcl-2
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TP53 protein, human
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Tumor Suppressor Protein p53
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bcl-2-Associated X Protein
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Transforming Growth Factors
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Ascorbic Acid