The pesticide concentration levels flowing into paddy fields and surrounding lagoons of the Rhône river delta were investigated over a period of 6 months in 2004. Water samples were collected at the outlets of the major ditches and in the lagoons in order to study the seasonal variation in pesticide concentrations and the spatial contamination profile. Twenty four pesticides were monitored, mainly herbicides and insecticides. Rice pesticides accounted for 90% of the detection rates while the pesticides transported by the Rhône river water dissolved phase only accounted for 10%. Pretilachlor, oxadiazon, MCPA and bentazone herbicides were found at the highest frequencies into the effluent waters of ditches with maximum concentration levels of 1.2, 0.8, 2.5 and 1.6 microg/L, respectively. Only one insecticide, tebufenozide, was sporadically detected at a maximum concentration level of 0.12 microg/L. There were two main peaks of contamination. The first one in April corresponded to the use of pre-emergence herbicides (oxadiazon and pretilachlor) and the second one in June was related to the post-emergence herbicides (MCPA and bentazone). These concentration peaks were well correlated with the pesticide application period time and rapid pesticide transfer (1-2 weeks) from fields to lagoons were observed. Increased loads of the pre-emergence herbicides were induced by the specific management of paddy fields which includes water emptying of fields before and after rice seeding. Pesticide dissipation into the lagoons occurred very quickly and the duration of the exposure of non-target aquatic organisms to high pesticide concentrations (in total a few microg/L level) was no longer than 2 weeks. According to the physico-chemical properties of the chemicals, contrasting results were observed when studying the spatial variation in pesticide concentrations through the lagoons. The concentrations of bentazone and MCPA, two substances with high phototransformation abilities, quickly decreased between the ditches and the lagoons while the oxadiazon and pretilachlor concentrations were more homogeneous.