Abstract
The Digene Hybrid Capture system cytomegalovirus (CMV) DNA (version 2.0), Roche CMV UL54 analyte-specific reagent, and QIAGEN RealArt CMV LightCycler PCR reagent tests were compared using whole-virus standards and plasma specimens collected from allogeneic-stem-cell transplant recipients. PCR assays showed better speed, sensitivity, and specificity.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Adult
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Child
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Child, Preschool
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Cytomegalovirus / genetics
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Cytomegalovirus / immunology
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Cytomegalovirus / isolation & purification*
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Cytomegalovirus Infections / diagnosis*
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Cytomegalovirus Infections / virology
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DNA, Viral* / analysis
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DNA, Viral* / blood
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DNA, Viral* / isolation & purification
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DNA-Directed DNA Polymerase / analysis
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DNA-Directed DNA Polymerase / genetics
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Humans
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Indicators and Reagents
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Polymerase Chain Reaction / methods*
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Reagent Kits, Diagnostic*
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Reproducibility of Results
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Sensitivity and Specificity
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Stem Cell Transplantation / adverse effects*
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Transplantation, Homologous / adverse effects*
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Viral Proteins / analysis
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Viral Proteins / genetics
Substances
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DNA, Viral
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Indicators and Reagents
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Reagent Kits, Diagnostic
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UL54 protein, Human herpesvirus 5
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Viral Proteins
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DNA-Directed DNA Polymerase