The importance of monolithic (continuous) beds is connected with their easy preparation and the far-reaching possibilities of modification of their surface and porous properties. These properties make them particularly attractive for the analysis of biologically important compounds characterized by a wide spectrum of physicochemical properties. This review summarizes their preparation methods as well as their application as continuous beds for determination of such biologically important compounds as catecholamines, vitamins, flavonoids, amino acids, peptides, and proteins.