Transient beta-glucuronidase expression in lily (Lilium longflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation

Biotechnol Lett. 2007 Jun;29(6):965-9. doi: 10.1007/s10529-007-9326-5. Epub 2007 Feb 20.

Abstract

A pollen-based transient expression system has been developed. Lily pollen grains, wounded by vigorous shaking in the presence of aluminum oxide particles, were transformed by infiltration with Agrobacterium tumefaciens LBA4404 cells harboring the beta-glucuronidase (GUS) gene construct, pBI121. In histochemical and fluorometric GUS analysis, the wounding processes allowed efficient transformation and, in cDNA blot hybridization, GUS mRNA synthesis was clearly detected. Lily pollen with appropriate wounds, therefore, can be used conveniently for the rapid production of recombinant proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agrobacterium tumefaciens / genetics*
  • Agrobacterium tumefaciens / growth & development
  • Gene Expression Regulation, Enzymologic
  • Glucuronidase / genetics*
  • Glucuronidase / metabolism
  • Histocytochemistry
  • Lilium / enzymology
  • Lilium / genetics*
  • Lilium / microbiology
  • Plants, Genetically Modified
  • Pollen / enzymology
  • Pollen / genetics*
  • Pollen / microbiology
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stress, Mechanical
  • Transformation, Genetic

Substances

  • RNA, Messenger
  • Glucuronidase