Proteasome function is required for activation of programmed cell death in heat shocked tobacco Bright-Yellow 2 cells

FEBS Lett. 2007 Mar 6;581(5):917-22. doi: 10.1016/j.febslet.2007.01.071. Epub 2007 Feb 6.

Abstract

To find out whether and how proteasome is involved in plant programmed cell death (PCD) we measured proteasome function in tobacco cells undergoing PCD as a result of heat shock (HS-PCD). Reactive oxygen species (ROS) production, cytochrome c levels and caspase-3-like protease activation were also measured in the absence or presence of MG132, a proteasome inhibitor. We show that proteasome activation occurs in early phase of HS-PCD upstream of the caspase-like proteases activation; moreover inhibition of proteasome function by MG132 results in prevention of PCD perhaps due to the prevention of ROS production, cytochrome c release and caspase-3-like protease activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / drug effects
  • Apoptosis / physiology*
  • Caspase 3 / metabolism
  • Cell Line
  • Cytochromes c / metabolism
  • Cytosol / metabolism
  • Heat-Shock Response
  • Hot Temperature
  • Leupeptins / pharmacology
  • Mitochondria / metabolism
  • Nicotiana / cytology*
  • Nicotiana / drug effects
  • Nicotiana / enzymology*
  • Protease Inhibitors / pharmacology
  • Proteasome Endopeptidase Complex / metabolism*
  • Reactive Oxygen Species / metabolism

Substances

  • Leupeptins
  • Protease Inhibitors
  • Reactive Oxygen Species
  • Cytochromes c
  • Caspase 3
  • Proteasome Endopeptidase Complex
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde