Mercury concentration in gallstones of 146 women (49 smoking, 97 non-smoking) and 24 men (11 smoking, 13 non-smoking) was determined employing Inductively Coupled Plasma - Atomic Emission Spectroscopy (ICP-AES). The possibility of mercury accumulation in concretions in the gallbladder was confirmed by the study. The changes of given elements were different in comparison for smoking and non smoking women and men. The research of mercury occurrence in gallstones shows the presence of higher concentration at smoking both women (0.16 microgHg/g) and men (0.22 microgHg/g) in comparison to non smoking women (0.12 microgHg/g) and men (0.06 microgHg/g).