A new system has been developed to assess human alertness and to alert the subject with acoustic stimulation in accordance with the assessed level of alertness. Dynamic characteristics of saccadic eye movement (saccade: SC) were used to calculate an alertness index. Digital signal processor was adopted for the calculation. The system was tested through eye tracking tasks. The results indicated that the developed system could awaken the subject by feeding sound back to the subject. Also, arousal reaction induced by the sound was visualized quantitatively by analyzing values of the alertness index after the stimulation. These results indicate applicability of the system not only to awakening device for accident prevention, but also to a tool for investigating effects of the stimulation.