Chemical double-mutant cycles: dissecting non-covalent interactions

Chem Soc Rev. 2007 Feb;36(2):172-88. doi: 10.1039/b603842p. Epub 2006 Nov 27.

Abstract

Thermodynamic double-mutant cycles and triple-mutant boxes are widely employed for the experimental quantification of non-covalent interactions and cooperative effects in proteins. This review describes the application of these powerful methodologies to the study of non-covalent interactions in synthetic systems.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acids / chemistry
  • Hydrogen Bonding
  • Hydrophobic and Hydrophilic Interactions
  • Models, Molecular*
  • Mutation*
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / genetics*
  • Static Electricity
  • Thermodynamics*

Substances

  • Amino Acids
  • Proteins