Hunter-killed red deer (n = 68; Cervus elaphus) harvested from the Italian provinces of Bologna (Emilia Romagna) and Pistoia (Tuscany) (44 degrees 00'N 11 degrees 00'E) from October 2001 to January 2002 were examined for protostrongylid larvae. Twenty-eight animals (41%) had protostrongylid larvae in feces, lungs, and inguinal and iliac lymph nodes. Of these 28 animals, 20 were adults (71%), four were yearlings (14%), and four were calves (14%). Shape, length, width, and the location to the nematodes were consistent with Elaphostrongylus cervi, which has not been previously reported in Italy.