SNP analysis is among the most contemporary method for personal identification in forensic genetics, especially in analyses of untypical cases and in studies of degraded DNA samples from crime scenes. This paper shows the results of Central Poland population studies carried out using minisequencing and SNP-pentaplex, which contains 5 biallelic loci rs2294067, rs2282160, rs2070764, rs2277216 and rs1063739. DNA fragments were amplified in one multiplex PCR reaction and SNPs were identified by the minisequencing method. The combined PD was 0.9895253125, which makes the pentaplex useful for forensic applications.