GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine

Mert Döşkaya; Mina Kalantari-Dehaghi; Craig M Walsh; Elzbieta Hiszczyńska-Sawicka; D Huw Davies; Philip L Felgner; Liza S Z Larsen; Richard H Lathrop; G Wesley Hatfield; Jessica R Schulz; Yüksel Gürüz; Frances Jurnak

doi:10.1016/j.vaccine.2006.10.060

GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine

Vaccine. 2007 Feb 26;25(10):1824-37. doi: 10.1016/j.vaccine.2006.10.060. Epub 2006 Nov 20.

Authors

Mert Döşkaya¹, Mina Kalantari-Dehaghi, Craig M Walsh, Elzbieta Hiszczyńska-Sawicka, D Huw Davies, Philip L Felgner, Liza S Z Larsen, Richard H Lathrop, G Wesley Hatfield, Jessica R Schulz, Yüksel Gürüz, Frances Jurnak

Affiliation

¹ Department of Physiology and Biophysics, University of California, Irvine, CA 92697, USA. jurnak@uci.edu

PMID: 17234306
DOI: 10.1016/j.vaccine.2006.10.060

Abstract

The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GRA1 protein vaccine results reveal significant increase in immune response and prolonged survival against acute toxoplasmosis compared to DNA vaccinations. Immune response and protection conferred by codon-optimized GRA1 DNA vaccine was slightly better than wild type GRA1 DNA vaccine.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Sequence
Animals
Antigens, Protozoan / genetics
Antigens, Protozoan / immunology*
Base Sequence
Disease Models, Animal
Female
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Recombinant Proteins / immunology
Toxoplasmosis / immunology
Toxoplasmosis / prevention & control*
Vaccines, DNA / genetics
Vaccines, DNA / immunology*
Vaccines, Subunit / immunology*

Substances

Antigens, Protozoan
GRA1 antigen
Recombinant Proteins
Vaccines, DNA
Vaccines, Subunit