Abstract
We investigated the efficacy of a synthetic Streptococcus pyogenes vaccine targeting two virulence factors using the Lipid Core Peptide (LCP) delivery system. BALB/c mice were immunised intranasally with LCPs containing peptides encompassing T-cell and B-cell epitopes of the conserved C-repeat region of the M protein (J8) or the fibronectin-binding repeats region (FNBR) of SfbI, or a combination formulation containing peptides representing both antigens. LCPs were co-administered with the TLR2/6 agonist MALP-2 as mucosal adjuvant. Humoral and cellular immune responses stimulated at systemic and mucosal levels were strongest in mice immunised with the dual antigen formulation. Mice were completely protected following a respiratory challenge with a lethal dose of a heterologous S. pyogenes strain, whereas there was 70% and 90% survival in mice immunised with LCP-J8 and LCP-FNBR, respectively. This is the first report demonstrating the elicitation of better protective immunity by a dual antigen component S. pyogenes vaccine.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adhesins, Bacterial / genetics
-
Adhesins, Bacterial / immunology*
-
Adjuvants, Immunologic / administration & dosage
-
Adjuvants, Immunologic / pharmacology
-
Administration, Intranasal
-
Animals
-
Antibodies, Bacterial / blood
-
Antigens, Bacterial / genetics
-
Antigens, Bacterial / immunology*
-
Bacterial Outer Membrane Proteins / genetics
-
Bacterial Outer Membrane Proteins / immunology*
-
Carrier Proteins / genetics
-
Carrier Proteins / immunology*
-
Disease Models, Animal
-
Epitopes, B-Lymphocyte / genetics
-
Epitopes, B-Lymphocyte / immunology
-
Epitopes, T-Lymphocyte / genetics
-
Epitopes, T-Lymphocyte / immunology
-
Leukocytes, Mononuclear / immunology
-
Lipopeptides
-
Mice
-
Mice, Inbred BALB C
-
Oligopeptides / administration & dosage
-
Oligopeptides / pharmacology
-
Streptococcal Infections / immunology
-
Streptococcal Infections / prevention & control*
-
Streptococcal Vaccines / administration & dosage
-
Streptococcal Vaccines / immunology*
-
Streptococcus pyogenes / genetics
-
Streptococcus pyogenes / immunology*
-
Vaccines, Subunit / administration & dosage
-
Vaccines, Subunit / immunology
-
Vaccines, Synthetic / administration & dosage
-
Vaccines, Synthetic / immunology
Substances
-
Adhesins, Bacterial
-
Adjuvants, Immunologic
-
Antibodies, Bacterial
-
Antigens, Bacterial
-
Bacterial Outer Membrane Proteins
-
Carrier Proteins
-
Epitopes, B-Lymphocyte
-
Epitopes, T-Lymphocyte
-
Lipopeptides
-
Oligopeptides
-
Streptococcal Vaccines
-
Vaccines, Subunit
-
Vaccines, Synthetic
-
fibronectin-binding proteins, bacterial
-
streptococcal M protein
-
macrophage stimulatory lipopeptide 2