Abstract
Long-term potentiation (LTP) is a persistent increase in the strength of synaptic transmission triggered by neuronal activity. Here, we submitted hippocampal slices to a perfusion of forskolin and IBMX, which induces a long-lasting LTP (>4 h) (L-LTP). We separated the proteins of the CA1 region by two-dimensional gel electrophoresis (2-DE). We then immunoblotted them using an anti-p-Tyr antibody. We found a protein whose tyrosine phosphorylation was unchanged 10 min after LTP induction but was dramatically increased after 1h, dropping back to its baseline after 4 h. This protein was identified as rabphilin using matrix-assisted laser desorption ionisation-time of flight mass spectrometry (MALDI-TOF MS). We also demonstrated that genistein, an inhibitor of tyrosine phosphorylation, prevented the development of the late phase of electrically-induced L-LTP. Our results suggest that rabphilin, a protein present in presynaptic terminals, could play a role in the late phase of L-LTP.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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1-Methyl-3-isobutylxanthine / pharmacology
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Animals
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Binding Sites / drug effects
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Binding Sites / physiology
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Colforsin / pharmacology
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Enzyme Inhibitors / pharmacology
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Genistein / pharmacology
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Hippocampus / drug effects
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Hippocampus / metabolism*
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Long-Term Potentiation / drug effects
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Long-Term Potentiation / physiology*
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Male
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Mass Spectrometry
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Mice
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Mice, Inbred C57BL
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Nerve Tissue Proteins / analysis
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Nerve Tissue Proteins / chemistry
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Nerve Tissue Proteins / metabolism*
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Neural Pathways / drug effects
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Neural Pathways / metabolism*
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Organ Culture Techniques
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Phosphodiesterase Inhibitors / pharmacology
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Phosphorylation / drug effects
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Presynaptic Terminals / drug effects
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Presynaptic Terminals / metabolism*
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Synaptic Transmission / drug effects
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Synaptic Transmission / physiology
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Tyrosine / metabolism
Substances
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Enzyme Inhibitors
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Nerve Tissue Proteins
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Phosphodiesterase Inhibitors
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rabphilin protein, mouse
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Colforsin
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Tyrosine
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Genistein
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1-Methyl-3-isobutylxanthine