Ten biomass samples from both municipal and industrial pilot and full scale submerged membrane bioreactors (MBRs) with mixed liquor suspended solids concentrations (MLSS) ranging from 7.2 to 30.2g L(-1) were studied at six air-flow rates (0.7, 1.3, 2.3, 3, 4.4 and 6m(3)m(-3)h(-1)). Statistical analyses were applied to identify the relative impacts of the various bulk biomass characteristics on oxygen transfer. Of the biomass characteristics studied, only solids concentration (correlated with viscosity), the carbohydrate fraction of the EPS (EPS(c)) and the chemical oxygen demand (COD) concentration of the SMP (SMP(COD)) were found to affect the oxygen transfer parameters k(L)a(20) (the oxygen transfer coefficient) and alpha-factor. The relative influence on k(L)a(20) was MLSS>aeration>EPS(c)>SMP(COD) and on alpha-factor was MLSS>SMP(COD)>EPS(c)>aeration. Both k(L)a(20) and alpha-factor increased with increasing aeration and EPS(c) and decreased with increasing MLSS and SMP(COD). MLSS was found to be the main parameter controlling the oxygen transfer.