Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin

Jillian G Rouse; Jianzhong Yang; Jessica P Ryman-Rasmussen; Andrew R Barron; Nancy A Monteiro-Riviere

doi:10.1021/nl062464m

Effects of mechanical flexion on the penetration of fullerene amino acid-derivatized peptide nanoparticles through skin

Nano Lett. 2007 Jan;7(1):155-60. doi: 10.1021/nl062464m.

Authors

Jillian G Rouse¹, Jianzhong Yang, Jessica P Ryman-Rasmussen, Andrew R Barron, Nancy A Monteiro-Riviere

Affiliation

¹ Center for Chemical Toxicology Research and Pharmacokinetics, Department of Clinical Sciences, North Carolina State University, 4700 Hillsborough Street, Raleigh, North Carolina 27606, USA.

PMID: 17212456
DOI: 10.1021/nl062464m

Abstract

Dermatomed porcine skin was fixed to a flexing device and topically dosed with 33.5 mg.mL-1 of an aqueous solution of a fullerene-substituted phenylalanine (Baa) derivative of a nuclear localization peptide sequence (Baa-Lys(FITC)-NLS). Skin was flexed for 60 or 90 min or left unflexed (control). Confocal microscopy depicted dermal penetration of the nanoparticles at 8 h in skin flexed for 60 and 90 min, whereas Baa-Lys(FITC)-NLS did not penetrate into the dermis of unflexed skin until 24 h. TEM analysis revealed fullerene-peptide localization within the intercellular spaces of the stratum granulosum.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acids / chemistry*
Fluorescein-5-isothiocyanate
Fullerenes / chemistry*
Microscopy, Confocal
Nanoparticles / chemistry*
Nuclear Localization Signals
Peptides / pharmacokinetics*
Skin Absorption*

Substances

Amino Acids
Fullerenes
Nuclear Localization Signals
Peptides
Fluorescein-5-isothiocyanate