ESI mass spectrometry and X-ray diffraction studies of adducts between anticancer platinum drugs and hen egg white lysozyme

Chem Commun (Camb). 2007 Jan 14:(2):156-8. doi: 10.1039/b611122j. Epub 2006 Oct 26.

Abstract

The interactions of cisplatin and its analogues, transplatin, carboplatin and oxaliplatin, with hen egg white lysozyme were analysed through ESI mass spectrometry, and the resulting metallodrug-protein adducts identified; the X-ray crystal structure of the cisplatin lysozyme derivative, solved at 1.9 A resolution, reveals selective platination of imidazole Nepsilon of His15.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Binding Sites
  • Carboplatin / chemistry
  • Carboplatin / metabolism
  • Chickens
  • Cisplatin / analogs & derivatives
  • Cisplatin / chemistry*
  • Cisplatin / metabolism
  • Crystallography, X-Ray
  • Egg White
  • Models, Molecular
  • Muramidase / chemistry*
  • Muramidase / metabolism
  • Organoplatinum Compounds / chemistry
  • Organoplatinum Compounds / metabolism
  • Oxaliplatin
  • Platinum Compounds / chemistry
  • Platinum Compounds / metabolism
  • Protein Binding
  • Protein Conformation
  • Spectrometry, Mass, Electrospray Ionization*

Substances

  • Organoplatinum Compounds
  • Platinum Compounds
  • Oxaliplatin
  • transplatin
  • Carboplatin
  • hen egg lysozyme
  • Muramidase
  • Cisplatin