Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells

Holger Erfle; Rainer Pepperkok

doi:10.1385/1-59745-165-7:155

Production of siRNA- and cDNA-transfected cell arrays on noncoated chambered coverglass for high-content screening microscopy in living cells

Methods Mol Biol. 2007:360:155-61. doi: 10.1385/1-59745-165-7:155.

Authors

Holger Erfle¹, Rainer Pepperkok

Affiliation

¹ Cell Biology and Cell Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.

PMID: 17172730
DOI: 10.1385/1-59745-165-7:155

Abstract

In this chapter, we provide a protocol for the production of transfected cell arrays in living mammalian cells on noncoated chambered coverglass for the systematic functional analyses of human genes by high-content screening microscopy. This method should facilitate drug target validation by small-interfering RNAs.

MeSH terms

Image Processing, Computer-Assisted
Microscopy / instrumentation*
Microscopy / methods*
Oligonucleotide Array Sequence Analysis / methods*
RNA, Small Interfering*
Surface Properties
Transfection

Substances

RNA, Small Interfering