[The detection of the human immunodeficiency virus antigen in the lymphoid cells of seropositive subjects]

A P Kozlov; A V Emel'ianov; M N Traugott; T V Kurbatova; A G Malykh; P V Kolotvina; I V Peresleni; T D Smirnova; V V Kasatkin; A S Simbirtsev

[The detection of the human immunodeficiency virus antigen in the lymphoid cells of seropositive subjects]

Vopr Virusol. 1991 May-Jun;36(3):219-22.

[Article in Russian]

Authors

A P Kozlov, A V Emel'ianov, M N Traugott, T V Kurbatova, A G Malykh, P V Kolotvina, I V Peresleni, T D Smirnova, V V Kasatkin, A S Simbirtsev

PMID: 1716392

Abstract

Cells from anti-HIV-positive persons were used in experiments for virus isolation. The RT-activity, viral antigen, nucleic acids, electron microscopic morphology, and infectivity were studied. The data presented allow a conclusion that the virus was isolated. These data confirmed the previous diagnosis of HIV infection.

Publication types

English Abstract

MeSH terms

Blotting, Northern
Blotting, Southern
Cell Line
Cells, Cultured / immunology
Cells, Cultured / microbiology
DNA, Viral / genetics
HIV Antigens / blood*
HIV Seropositivity / immunology*
HIV Seropositivity / microbiology
HIV-1 / genetics
HIV-1 / immunology*
HIV-1 / ultrastructure
Humans
Lymphocytes / immunology*
Lymphocytes / microbiology
Microscopy, Electron
Nucleic Acid Hybridization
RNA, Viral / genetics
RNA-Directed DNA Polymerase / analysis
Virion / ultrastructure

Substances

DNA, Viral
HIV Antigens
RNA, Viral
RNA-Directed DNA Polymerase