Purpose: To establish the mutant Myocilin gene plasmid in order to study the function of Myocilin.
Methods: The mutant site was induced by site-directed mutagenesis. This plasmid were transfected into human trabecular meshwork cells (HTM cells) by cationic liposomes and the expression of Myocilin was examined by RT-PCR and Western blot analysis.
Results: The mutant plasmid were correctly constructed. Myocilin was efficiently expressed in HTM cells transfected with this plasmid.
Conclusion: The constructed eukaryote expression plasmid pcDNA-MYOC-P370L could express Myocilin in HTM cells in vitro.