RNA isolation using immobilized PNA

Takashi Ohtsuki; Chisato Kumano; Taishi Manabe; Mizuki Kitamatsu; Masahiko Sisido

doi:10.1093/nass/49.1.263

RNA isolation using immobilized PNA

Nucleic Acids Symp Ser (Oxf). 2005:(49):263-4. doi: 10.1093/nass/49.1.263.

Authors

Takashi Ohtsuki¹, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Affiliation

¹ Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.

PMID: 17150734
DOI: 10.1093/nass/49.1.263

Abstract

A novel method to isolate a single kind of RNA from RNA mixture was developed by using a biotinylated-PNA (peptide nucleic acid) that is complementary to the RNA. Efficient bindings of Escherichia coli tRNA(Leu)CUC were observed to the 9-mer and 12-mer PNAs that are complementary to the 3'-end region of the tRNA. Purified E. coli tRNA(Leu)CUC was obtained from E. coli tRNA mixture.

MeSH terms

Base Sequence
Biotinylation
Escherichia coli / genetics
Molecular Sequence Data
Peptide Nucleic Acids / chemistry*
RNA, Bacterial / chemistry
RNA, Bacterial / isolation & purification
RNA, Transfer / isolation & purification*
RNA, Transfer, Leu / chemistry
RNA, Transfer, Leu / isolation & purification

Substances

Peptide Nucleic Acids
RNA, Bacterial
RNA, Transfer, Leu
RNA, Transfer