[Adriamycin enhances anti-human DR5 monoclonal antibody (mDRA-6) induced HL-60 cells apoptosis]

Zhonghua Xue Ye Xue Za Zhi. 2006 Jul;27(7):461-4.
[Article in Chinese]

Abstract

Objective: To investigate synergistic killing effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody (mDRA-6) and adriamycin(Adr) on HL-60 cells.

Methods: mDRA-6 was prepared by immunizing BALB/c mice with DR5 protein. DR5 expression on Adr-treated HL-60 cells was detected by flow cytometry. Morphologic changes of HL-60 cells were observed under fluorescence microscope. Cytotoxic and apoptotic effects of mDRA-6 and Adr on HL-60 cells were measured by MTT analysis. DNA fragmentation was detected by agarose gel electrophoresis.

Results: Adr induce DR5 expression on HL-60 cells. Cell budding, chromatin condensation and apoptotic body formation were observed in HL-60 cells treated by mDRA-6 and Adr. Death and apoptosis of these cells and DNA ladder were exhibited on agarose gel electrophoresis.

Conclusion: mDRA-6 and Adr have synergistic killing effect on HL-60 cells.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Apoptosis / drug effects*
  • Dose-Response Relationship, Drug
  • Doxorubicin / pharmacology*
  • HL-60 Cells
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Receptors, TNF-Related Apoptosis-Inducing Ligand / immunology*
  • TNF-Related Apoptosis-Inducing Ligand / immunology

Substances

  • Antibodies, Monoclonal
  • Receptors, TNF-Related Apoptosis-Inducing Ligand
  • TNF-Related Apoptosis-Inducing Ligand
  • Doxorubicin